图象采集实例
[attach]2486[/attach]The program is designed for coordinating image acquisition using the Qimaging XOP (WaveMetrics) and Qimaging Retiga Exi Fast 1394 monochrome cooled CCD camera ([url=http://www.qimaging.com/]www.Qimaging.com[/url]) with a laser combiner (for a HeCd and Ar ion laser providing laser lines of 442, 488, and 514 nm) manufactured by Prairie Technologies ([url=http://www.prairie-technologies.com/]www.prairie-technologies.com[/url]). The laser intenstiy and "shuttering" are controlled through a NEOS acoustic optical tunable filter using an Instrutech ITC-18 data acquisition board ([url=http://www.instrutech.com/]www.Instrutech.com[/url]). The ITC-18 is controlled by the ITC18 XOP furnished by Instrutech. The eventual goal is to add patch-clamp electronphysiological data acquisition to the program using the ITC-18. The setup is designed to acquire fluorescence resonsance energy transfer (FRET) imaging data using Total Internal Reflectance Fluorescence (TIRF) imaging modality on an Olympus IX71 inverted microscope equipped with a 60x 1.45 na objective.
Submitted by
Stephen R. Ikeda, M.D., Ph.D.
Chief, Lab. of Molecular Physiology
National Institutes of Health
National Institute on Alcohol Abuse and Alcoholi
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